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Activities of overt and latent diacylglycerol acyltransferases (DGATs I and II) in liver microsomes of ob/ob mice

International Journal of Obesity volume 26, pages 742–743 (2002)Cite this article

Abstract

The effect of the ob/ob mutation on microsomal overt and latent acyl CoA:diacylglycerol acyl transferase activities (DGATs I and II) in liver microsomes of mice was investigated. Overt and latent hepatic DGAT activities for 16-week-old lean Ob/? mice were 0.42±0.14 and 1.57±0.04 µmol/mg/min, respectively. For ob/ob mice DGAT I and II activities were 1.17±0.28 and 3.09±0.78 µmol/mg/min, respectively. The hepatic triacylglycerol (TAG) concentration of 16-week-old mice was increased three-fold in ob/ob mice relative to those in lean controls. The data suggest that the increased rate of hepatic TAG secretion and intracellular accumulation in ob/ob mice is accompanied by parallel increases in the activities of both DGATs.

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Acknowledgements

We thank Dr A Sørrenson for provision of the mice. This work was supported by the British Heart Foundation.

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  1. Cell Biochemistry, Hannah Research Institute, Ayr, Scotland, UK

    IJ Waterman & VA Zammit

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  1. IJ Waterman
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  2. VA Zammit
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Correspondence to IJ Waterman.

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Waterman, I., Zammit, V. Activities of overt and latent diacylglycerol acyltransferases (DGATs I and II) in liver microsomes of ob/ob mice. Int J Obes 26, 742–743 (2002). https://doi.org/10.1038/sj.ijo.0801981

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