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Further evidence for the association between obesity-related traits and the apolipoprotein A-IV gene

International Journal of Obesity volume 27, pages 484–490 (2003)Cite this article

Abstract

OBJECTIVE: To investigate associations and gene–environment interactions of APOA-IV gene polymorphisms with obesity-related phenotypes in a Brazilian population.

METHODS: A total of 391 individuals (171 men and 220 women) were genotyped for Xbal, Thr347Ser and Gln360His polymorphisms by PCR-RFLP methods. Adjusted body mass index (BMI) and waist circumference (WC) were compared among genotypes/haplotypes by unpaired t-test or analysis of variance. Gene–environment interactions were tested by analysis of variance using a general linear model.

RESULTS: Analysis of the APOA-IV gene variants separately showed that X*2 and 347Ser alleles were associated with higher BMI (P=0.02 for both polymorphisms). Haplotype analysis confirmed this association. For these polymorphisms, the effect on BMI appeared to depend on smoking status (test for interaction, P=0.007 and 0.02, respectively), the Thr347Ser variant was associated with a BMI increase in smokers only (P=0.002). At the single-locus level no association was observed between 360His allele and BMI; however, haplotype analyses showed an association of this gene variant and higher BMI. A trend for association with WC (P=0.05) was observed in male carriers of the 360His allele. The effect of this polymorphism also depended on smoking status (test for interaction, P=0.018). Nonsmoker male carriers of the 360His allele had a larger waist circumference than homozygotes for the Gln allele (P=0.003).

CONCLUSION: Our data suggest that the APOA-IV gene polymorphisms investigated are associated with obesity-related traits. The effects of X*2 and 347Ser variants on BMI and the 360His variant on waist circumference depended on smoking status.

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Acknowledgements

Thanks are due to Ana Lúcia S Antunes and Maria Perpétua de O Pinto from the Clinical Analysis Laboratory of the Pharmacy College and to Gledison Gastaldo from the Biochemical Laboratory of the Clinical Hospital of Porto Alegre. We are also grateful to André Vargas, Fabiana M de Andrade, Marsel Arsand, Silvana de Almeida and Vanessa S Mattevi for help in sample collection. Financial support was provided by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil), Programa de Apoio a Núcleos de Excelência (PRONEX, Brazil) and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS, Brazil).

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  1. Genetics Department, Federal University of Rio Grande do Sul, Porto Alegre, Brazil

    M Fiegenbaum & M H Hutz

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  1. M Fiegenbaum
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Correspondence to M H Hutz.

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Fiegenbaum, M., Hutz, M. Further evidence for the association between obesity-related traits and the apolipoprotein A-IV gene. Int J Obes 27, 484–490 (2003). https://doi.org/10.1038/sj.ijo.0802256

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