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Allelotyping defines minimal imbalance at chromosomal region 17q25 in non-serous epithelial ovarian cancers

Oncogene volume 19, pages 1466–1472 (2000)Cite this article

Abstract

Allelic deletions of multiple chromosome 17q loci in sporadic ovarian cancer of epithelial origin suggest that inactivation of tumor suppressor gene(s) in these regions may be important for ovarian tumorigenesis. To further define the pattern of allelic imbalance in epithelial ovarian tumors of different histologies, a PCR-based assay was used to assess loss of heterozygosity (LOH) of polymorphic markers representative of TP53, BRCA1, NME1 and GH1, and region 17q23-25. LOH was observed for at least one marker in 68% of malignant tumors (n=60) and in 18% tumors of borderline malignancy (n=11), but not in benign tumors (n=5). The highest frequency of LOH in malignant tumors (64%) was observed with D17S801 on 17q25. Ten of 39 malignant ovarian tumors displaying LOH of at least one 17q marker, displayed a LOH pattern enabling the determination of a minimal region of overlapping deletion defined by D17S795 and D17S801. One borderline tumor also displayed an interstitial LOH pattern that overlapped this 17q25 minimal region of deletion. The histologies of malignant tumors displaying a pattern indicative of interstitial 17q deletions were of the endometrioid, clear cell and mucinous epithelial types. As the minimal region of overlap defined by these tumors overlap regions deleted in malignant tumors of all histologic types, and in a tumor of borderline malignancy, the 17q25-tumor suppressor may be implicated in the development of all types of epithelial ovarian tumors.

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Acknowledgements

We thank L Champoux and N Belanger for technical and clerical assistance. We are grateful to Drs A Mehio, P Drouin, P Audet-Lapointe, J Dubuc-Lissoir, P Gauthier and L Rinet for providing clinical specimens and pathology reports. We thank Dr Tom Hudson for helpful comments. This research was funded by research grants from the Medical Research Council of Canada and National Cancer Institute of Canada to PN Tonin, A-M Mes-Masson and D Provencher. A-M Mes-Masson is the recipient of a senior Fonds de Recherche en Santé du Québec fellowship. PN Tonin is a Cancer Research Society-Medical Research Council of Canada scholar.

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Authors and Affiliations

  1. Montreal General Hospital Research Institute, 1650 Cedar Avenue, Montreal, Quebec, H3G 1A4, Canada

    France Dion & Patricia N Tonin

  2. Institut du cancer de Montréal/Centre Hospitalier Université de Montréal (CHUM) – Hôpital Notre-Dame, 1560 rue Sherbrooke Est, Montréal, Québec, H2L 4M1, Canada

    Anne-Marie Mes-Masson & Diane Provencher

  3. Département de Médecine, Université de Montréal, 6128 succursale Centre-ville, Montréal, Québec, H3C 3J2, Canada

    Anne-Marie Mes-Masson

  4. Deparment of Obstetrics and Gynecology, Montreal General Hospital, 1650 Cedar Avenue, Montreal, Quebec, H3G 1A4, Canada

    Robert J Seymour

  5. Département d'Obstétrique-gynécologie, Université de Montréal, 6128 succursale Centre-ville, Montréal, Québec, H3C 3J2, Canada

    Diane Provencher

  6. Department of Human Genetics, McGill University, 1205 Docteur Penfield Avenue, Montreal, Quebec, H3A 1B1, Canada

    Patricia N Tonin

  7. Department of Medicine, McGill University, 1205 Docteur Penfield Avenue, Montreal, Quebec, H3A 1B1, Canada

    Patricia N Tonin

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  1. France Dion
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Dion, F., Mes-Masson, AM., Seymour, R. et al. Allelotyping defines minimal imbalance at chromosomal region 17q25 in non-serous epithelial ovarian cancers. Oncogene 19, 1466–1472 (2000). https://doi.org/10.1038/sj.onc.1203463

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