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  • Original Paper
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Tumorigenesis mediated by MET mutant M1268T is inhibited by dominant-negative Src

Oncogene volume 19, pages 2996–3002 (2000)Cite this article

Abstract

We recently described germline and somatic mutations in the MET gene associated with papillary renal carcinoma type 1. MET mutation M1268T was located in a codon highly conserved among receptor tyrosine kinases, and homologous to the codon mutated in multiple endocrine neoplasia type 2B, and many cases of sporadic medullary carcinoma of the thyroid gland (Ret M918T). Ret M918T and MET M1268T have previously been shown to be highly active in mouse NIH3T3 transformation assays, and to change the substrate specificity of the kinase. We studied the mechanism of transformation mediated by MET M1268T by analysing a clone, F4, derived from NIH3T3 cells transformed by MET M1268T. In contrast to NIH3T3 cells, F4 cells grew in suspension in tissue culture, and rapidly formed tumors in nude mice. We found that c-Src was constitutively bound to MET proteins in F4 cells, and that Src kinase activity was elevated. Transfection of dominant negative Src constructs into F4 cells eliminated the ability of F4 cells to grow in suspension culture and retarded the growth of F4 cells in vivo. The ability of transfected dominant negative Src constructs to inhibit the growth of F4 cells correlated with the inhibition of phosphorylation of paxillin and focal adhesion kinase. Transfection of dominant negative Src constructs into F4 cells had no effect on Grb2 binding or PLC γ phosphorylation. The results suggest that c-Src participates in the tumorigenic phenotype induced in NIH3T3 cells by MET M1268T by signaling through focal adhesion kinase and paxillin.

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Acknowledgements

We thank Mike Jeffers and George Vande Woude for helpful discussion and for MET expression vector, Alla Danilkovitch for the critical reading of manuscript, Kenneth B Kaplan for dominant negative Src expression vectors, and W Gregory Alvord for statistical study. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government. This project has been funded in part with Federal Funds from the National Cancer Institute, National Institutes of Health, under Contract No. NO1-CO-56000. The publisher or recipient acknowledges right of the US Government to retain a nonexclusive, royalty-free license in and to any copyright covering the article.

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Authors and Affiliations

  1. Laboratory of Immunobiology, NCI-Frederick Cancer Research and Development Center, Frederick, 21702, Maryland, MD, USA

    Noboru Nakaigawa, Gregor Weirich & Berton Zbar

  2. Intramural Research Support Program (LS), SAIC Frederick, National Cancer Institute—Frederick Cancer Research and Development Center, Frederick, 21702, Maryland, MD, USA

    Laura Schmidt

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  1. Noboru Nakaigawa
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Nakaigawa, N., Weirich, G., Schmidt, L. et al. Tumorigenesis mediated by MET mutant M1268T is inhibited by dominant-negative Src. Oncogene 19, 2996–3002 (2000). https://doi.org/10.1038/sj.onc.1203628

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