Figure 3 | Oncogene

Figure 3

From: PTEN expression is reduced in a subset of sporadic thyroid carcinomas: evidence that PTEN-growth suppressing activity in thyroid cancer cells is mediated by p27kip1

Figure 3

Expression of PTEN in normal and neoplastic thyroid tissues. (a) Northern blot analysis. Total RNA (20 μg) were size-fractionated on denaturing gel, transferred to nitrocellulose and hybridized with PTEN cDNA. Lane TN, normal thyroid tissue. Lanes 1–18: thyroid carcinomas. (b) Western blot analysis of PTEN expression in thyroid tumor-derived cell lines. Fifty micrograms of total proteins were resolved on 10% SDS–PAGE, transferred to nitrocellulose filters and probed with anti-PTEN antibodies. Antibodies to α-tubulin were used as loading control. Normal thyroid tissue, lane TN; papillary carcinomas, lanes 2, 3, 5, 6 and 7; follicular carcinomas: lane 4; anaplastic carcinomas: lanes 8 and 9

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