Figure 4 | Oncogene

Figure 4

From: PTEN expression is reduced in a subset of sporadic thyroid carcinomas: evidence that PTEN-growth suppressing activity in thyroid cancer cells is mediated by p27kip1

Figure 4

Re-expression of PTEN in thyroid tumor cells inhibits colony outgrowth. (a) The full-length PTEN cDNA was cloned into a pCMV vector. (b) Expression of exogenous PTEN cDNA into ARO, TPC-1 or FB-1 cells was detected by RT–PCR using SP6/1010 (upper panel) or 320/640 (middle panel) internal primer pairs on total RNA extracted from cells transiently transfected by pCMV (lanes 1, 3 and 5) or pCMV-PTEN (lanes 2, 4 and 6), respectively. Primers to amplify the aldolase transcript were used as internal control (lower panel). (c) Colony assay. Exponentially growing FB-1 cells were transfected either with the pCMV-PTEN or with a control pCMV plasmid, selected with G418, and stained with crystal violet

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