Figure 5 | Oncogene

Figure 5

From: PTEN expression is reduced in a subset of sporadic thyroid carcinomas: evidence that PTEN-growth suppressing activity in thyroid cancer cells is mediated by p27kip1

Figure 5

p27 up-regulation is necessary for PTEN-dependent growth arrest. (a) BrdU incorporation of pFLAG- or pFLAG-PTEN-transfected FB-1 cells. First column: one green pFLAG-transfected cell incorporates BrdU (yellow arrow). Second column: one green pFLAG-PTEN-expressing cell does not incorporate BrdU (red arrow). Third column: one green pFLAG-PTEN-expressing cell incorporates BrdU in the presence of antisense p27kip1 plasmid (yellow arrow). A 40×Neo-Achromat Zeiss objective was used. (b) Statistical analysis of BrdU incorporation in pEGF-PTEN-transfected FB-1 cells. (c) Western blot analysis with α-EGFP, α-Ser473-Akt, α-p27kip1 antibodies of transfected FB-1 cells. Lane 1, pEGFP-transfected cells; lane 2, pEGFP-PTEN-transfected cells; lane 3, pEGFP-PTEN-transfected cells in the presence of an excess antisense p27kip1 plasmid

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