Figure 6 | Oncogene

Figure 6

From: PTEN expression is reduced in a subset of sporadic thyroid carcinomas: evidence that PTEN-growth suppressing activity in thyroid cancer cells is mediated by p27kip1

Figure 6

Analysis of PTEN expression, p27 expression and Akt phosphorylation in human thyroid tumors and cell lines. (a) Western blot analysis of PTEN, p27kip1 and Akt expression and Ser474 phosphorylation in thyroid carcinomas. Fifty micrograms of total proteins were resolved on 10% SDS–PAGE, transferred to nitrocellulose filters and probed with anti-PTEN antibodies (first panel), anti-p27kip1 (second panel), anti-Ser473-specific antibodies (third panel) and anti-Akt antibodies (lower panel). (b) Western blot analysis of PTEN, p27kip1 and Akt expression and Ser474 phosphorylation in thyroid carcinoma-derived cell lines. Fifty micrograms of total proteins were resolved on 10% SDS–PAGE, transferred to nitrocellulose filters and probed with anti-PTEN (first panel), anti-p27kip1 antibodies (second panel), anti-Ser473-specific antibodies (middle panel) and anti-Akt antibody (lower panel)

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