Figure 1
A transient increase in endogenous ubiquitin conjugates upon exposure to NCS. Lymphoblastoid cells from a normal (L-40) and an A-T patient (A-T59) at a concentration of 0.5×106 cells/ml were treated with 80 ng/ml of NCS for 0–4 h. Endogenous ubiquitin conjugates were detected by Western blotting analysis of cellular extracts using an anti-ubiquitin antiserum. The membrane was reprobed using anti-actin antibody to monitor loads of the different lanes in the gel. (a) Wild type cells; (b) cells derived from an A-T patient. (c and d) Present densitometric analyses of the data in a and b, respectively. The data in c and d are the ratios relative to actin of the optical density of the area of the film at which ubiquitin conjugates are observed in the Western blot. The ratio was designated as 100 for the cells at time 0
