Figure 3
A transient increase in endogenous ubiquitin conjugates upon treatment with H2O2. Lymphoblastoid cells from a normal (NL553) and an A-T patient (A-T59) were treated with a single bolus of 500 μM for 0–8 h. Cells were collected at time 0, 0.5, 2, 4 and 8 h. Endogenous ubiquitin conjugates were determined by Western blot analysis using an anti-ubiquitin antiserum. To normalize for any potential differences in protein loading, the blots were stripped and reprobed with an anti-actin antibody. (a) Normal cells; (b) A-T cells. (c) Comparison of endogenous ubiquitin conjugates between wild type cells and A-T cells at time 0 and 0.5 h after H2O2 exposure. (d) Densitometric analyses of data in c, in which ubiquitin conjugate levels were normalized to actin levels
