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A monoclonal antibody specific for BK virus large T-antigen (clone BK.T-1) also binds the human Ku autoantigen

Oncogene volume 21, pages 5725–5732 (2002)Cite this article

Abstract

A monoclonal antibody directed against the BK virus large T-antigen (clone BK.T-1) has previously been used to evaluate BKV-T antigen (BKV-TAg) expression. However, our experience showed a consistent reactivity to uninfected human, but not rodent cells and tissues. Using immunoprecipitation, Western analysis, amino acid sequencing and end-point dilution analysis, we analysed the BK.T-1 antibody reactivity and identified the bound cellular protein. The results clearly show that the antibody recognizes the large subunit (Ku86) of the Ku autoantigen, the regulatory component of the DNA-PKcs. We also demonstrated that the antibody retained its original reactivity in BKV-TAg transformed hamster kidney cells. The cross-reaction of the BK.T-1 antibody suggests a possible similarity between BKV-TAg and Ku86, but makes the antibody unsuitable for studies of BKV large T-antigen in the human cells.

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References

  • Cai QQ, Plet A, Imbert J, Lafage-Pochtaloff M, Cerdan C, Blanchard JM . 1994 Cytogenet. Cell. Genet. 65: 221–227

  • Fanning E, Knippers R . 1992 Ann. Rev. Biochem. 61: 55–85

  • Flaegstad T, Andersen PA, Johnsen JI, Asomani SK, Jorgensen GE, Vignarajan S, Kjuul A, Kogner P, Traavik T . 1999 Cancer Res. 59: 1160–1163

  • Gu Y, Seidl KJ, Rathbun GA, Zhu C, Manis JP, van der Stoep N, Davidson L, Cheng HL, Sekiguchi JM, Frank K, Stanhope-Baker P, Schlissel MS, Roth DB, Alt FW . 1997 Immunity 7: 653–665

  • Imperiale MJ . 2000 Virology 267: 1–7

  • Jin S, Weaver DT . 1997 EMBO J. 16: 6874–6885

  • Le Romancer M, Reyl-Desmars F, Cherifi Y, Pigeon C, Bottari S, Meyer O, Lewin MJ . 1994 J. Biol. Chem. 269: 17464–17468

  • Li GC, Ouyang H, Li X, Nagasawa H, Little JB, Chen DJ, Ling CC, Fuks Z, Cordon-Cardo C . 1998 Mol. Cell. 1: 1–8

  • Marshall J, Smith A, Cheng SH . 1991 Oncogene 6: 1673–1676

  • Meneguzzi C, Chenciner N, Corallini A, Grossi MP, Barbanti-Brodano G . 1981 Virology 111: 139–153

  • Messier H, Fuller T, Mangal S, Brikner H, Igarashi S, Gaikwad J, Fotedar R, Fotedar A . 1993 Proc. Natl. Acad. Sci. USA 90: 2685–2689

  • Nussenzweig A, Chen C, da Costa Soares V, Sanchez M, Sokol K, Nussenzweig MC, Li GC . 1996 Nature 382: 551–555

  • Ochem AE, Skopac D, Costa M, Rabilloud T, Vuillard L, Simoncsits A, Giacca M, Falaschi A . 1997 J. Biol. Chem. 272: 29919–29926

  • Shinohara T, Matsuda M, Cheng SH, Marshall J, Fujita M, Nagashima K . 1993 J. Med. Virol. 41: 301–305

  • Simmons DT, Takemoto KK, Martin MA . 1978 Virology 85: 137–145

  • Zhu C, Bogue MA, Lim DS, Hasty P, Roth DB . 1996 Cell 86: 379–389

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Acknowledgements

We are grateful to S Hirst for her excellent technical assistance and to Dr A Simoneau from the Department of Urology, VA Hospital, Long Beach, California, USA, for providing the prostate specimens. The work was supported by California Cancer Research Program grant to L Villarreal.

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  1. Department of Molecular Biology and Biochemistry, University of California at Irvine, Irvine, 92697, California, CA, USA

    Alberto Zambrano & Luis P Villarreal

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  1. Alberto Zambrano
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  2. Luis P Villarreal
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Correspondence to Luis P Villarreal.

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Zambrano, A., Villarreal, L. A monoclonal antibody specific for BK virus large T-antigen (clone BK.T-1) also binds the human Ku autoantigen. Oncogene 21, 5725–5732 (2002). https://doi.org/10.1038/sj.onc.1205692

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