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Binding of the corepressor TLE1 to Qin enhances Qin-mediated transformation of chicken embryo fibroblasts

Oncogene volume 22, pages 1749–1757 (2003)Cite this article

Abstract

The oncoprotein Qin is a member of the winged helix family of transcriptional regulators. The region C-terminal to its winged helix DNA-binding domain is required for transformation of chicken embryo fibroblasts. We isolated the corepressor TLE1 as a binding partner for Qin in a yeast two-hybrid screen and localized the TLE1-binding region to a 60 amino-acid stretch directly C-terminal of the winged helix domain of Qin. We show in vivo interaction of full-length Qin and TLE1 in a mammalian two-hybrid system. Coexpression of TLE1-binding Qin and TLE1 induces phosphorylation of TLE1. The DNA-binding activity of Qin is not required for this function. Binding of Qin to TLE1 correlates with Qin-induced transformation. Addition of the TLE1-binding motif WRPW to the C-terminus of a transformation-defective Qin deletion mutant restores binding to TLE1 and significantly enhances transformation. Expression of TLE1 in CEF by the retroviral vector RCAS enhances cell growth and induces formation of agar colonies.

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References

  • Aoki M . (2002). Personal communication.

  • Aoki M, Batista O, Bellacosa A, Tsichlis P and Vogt PK . (1998). Proc. Natl. Acad. Sci. USA, 95, 14950–14955.

  • Aoki M, Schetter C, Himly M, Batista O, Chang HW and Vogt PK . (2000). J. Biol. Chem., 275, 6267–6275.

  • Artavanis-Tsakonas S, Rand MD and Lake RJ . (1999). Science, 284, 770–776.

  • Chang HW, Li J and Vogt PK . (1996). Oncogene, 13, 441–444.

  • Chen G and Courey AJ . (2000). Gene, 249, 1–16.

  • Chen G, Fernandez J, Mische S and Courey AJ . (1999). Genes Dev., 13, 2218–2230.

  • Chen G, Nguyen PH and Courey AJ . (1998). Mol. Cell. Biol., 18, 7259–7268.

  • Choi CY, Kim YH, Kwon HJ and Kim Y . (1999). J. Biol. Chem., 274, 33194–33197.

  • Dubnicoff T, Valentine SA, Chen G, Shi T, Lengyel JA, Paroush Z and Courey AJ . (1997). Genes Dev, 11, 2952–2957.

  • Duff RG and Vogt PK . (1969). Virology, 39, 18–30.

  • Eberhard D, Jimenez G, Heavey B and Busslinger M . (2000). EMBO J, 19, 2292–2303.

  • Gietz RD, Triggs-Raine B, Robbins A, Graham KC and Woods RA . (1997). Mol. Cell. Biochem., 172, 67–79.

  • Husain J, Lo R, Grbavec D and Stifani S . (1996). Biochem. J., 317, 523–531.

  • Imai Y, Kurokawa M, Tanaka K, Friedman AD, Ogawa S, Mitani K, Yazaki Y and Hirai H . (1998). Biochem. Biophys. Res. Commun., 252, 582–589.

  • Kawai S and Nishizawa M . (1984). Mol. Cell. Biol., 4, 1172–1174.

  • Levanon D, Goldstein RE, Bernstein Y, Tang H, Goldenberg D, Stifani S, Paroush Z and Groner Y . (1998). Proc. Natl. Acad. Sci. USA, 95, 11590–11595.

  • Li J, Chang HW, Lai E, Parker EJ and Vogt PK . (1995). Cancer Res., 55, 5540–5544.

  • Li J, Thurm H, Chang HW, Iacovoni JS and Vogt PK . (1997). Proc. Natl. Acad. Sci. USA, 94, 10885–10888.

  • Liu Y, Dehni G, Purcell KJ, Sokolow J, Carcangiu ML, Artavanis-Tsakonas S and Stifani S . (1996). Genomics, 31, 58–64.

  • Ma Y, Geerdes DW and Vogt PK . (2000). Oncogene, 19, 4815–4821.

  • Midorikawa Y, Tsutsumi S, Taniguchi H, Ishii M, Kobune Y, Kodama T, Makuuchi M and Aburatani H . (2002). Jpn. J. Cancer Res., 93, 636–643.

  • Minucci S, Maccarana M, Cioce M, De Luca P, Gelmetti V, Segalla S, Di Croce L, Giavara S, Matteucci C, Gobbi A, Bianchini A, Colombo E, Schiavoni I, Badaracco G, Hu X, Lazar MA, Landsberger N, Nervi C and Pelicci PG . (2000). Mol. Cell., 5, 811–820.

  • Nishizawa M, Fu SL, Kataoka K and Vogt PK . (2002). submitted.

  • Nuthall HN, Husain J, McLarren KW and Stifani S . (2002). Mol. Cell. Biol., 22, 389–399.

  • Palaparti A, Baratz A and Stifani S . (1997). J. Biol. Chem., 272, 26604–26610.

  • Sonderegger CK, Narisawa-Saito M and Vogt PK . (2003). Oncogene, in press.

  • Stifani S, Blaumueller CM, Redhead NJ, Hill RE and Artavanis-Tsakonas S . (1992). Nat. Genet., 2, 119–127.

  • Weinmaster G . (1997). Mol. Cell. Neurosci., 9, 91–102.

  • Yao J, Lai E and Stifani S . (2001). Mol. Cell. Biol., 21, 1962–1972.

  • Yao J, Liu Y, Husain J, Lo R, Palaparti A, Henderson J and Stifani S . (1998). Dev. Growth Differ., 40, 133–146.

  • Yao J, Liu Y, Lo R, Tretjakoff I, Peterson A and Stifani S . (2000). Mech. Dev., 93, 105–115.

  • Zhang J, Hug BA, Huang EY, Chen CW, Gelmetti V, Maccarana M, Minucci S, Pelicci PG and Lazar MA . (2001). Mol. Cell. Biol., 21, 156–163.

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Acknowledgements

We thank Martin Sonderegger for technical assistance. This work was supported by United States Public Health Service Grants from the National Cancer Institute. CKS was supported by Schroedinger fellowships J1691-GEN and J1874-GEN from the Austrian Science Foundation FWF Austria. This is manuscript number 14969-MEM at The Scripps Research Institute. We thank S Stifani for a kind gift of TLE1.

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  1. Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, 92037, CA, USA

    Corinna K Sonderegger & Peter K Vogt

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  1. Corinna K Sonderegger
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  2. Peter K Vogt
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Correspondence to Peter K Vogt.

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Sonderegger, C., Vogt, P. Binding of the corepressor TLE1 to Qin enhances Qin-mediated transformation of chicken embryo fibroblasts. Oncogene 22, 1749–1757 (2003). https://doi.org/10.1038/sj.onc.1206308

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