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  • Oncogenomics
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MLL-mediated transcriptional gene regulation investigated by gene expression profiling

Oncogene volume 22, pages 3655–3668 (2003)Cite this article

Abstract

The human mixed lineage leukemia (MLL) gene is involved in about 50 different chromosomal translocations, associated with the disease phenotype of acute leukemia. However, the normal function of MLL is less understood. Homozygous knockouts of murine Mll were embryonal lethal, while heterozygous disruption led to aberrant hox gene expression associated with skeletal malformations, growth retardation, and impaired hematopoiesis. To understand MLL functions on the molecular level, gene expression profiling experiments were performed with a pair of murine cell lines (MLL+/+ and MLL−/−). Microarray hybridization experiments revealed 197 potential target genes that are differentially expressed, providing new and important clues about MLL functions.

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Acknowledgements

We thank Dr Jay Hess for providing the MLL wild-type and knockout cell 1ines, Dr Peter Oliver for a critical reading of the manuscript, and Dr Ludger Klein-Hitpass for Affymetrix chip hybridzation and computer analysis. This work was funded in part by the DFG-Grant MA 1876/3-1 and the BMBF cancer network Grant 01GS0104/K-S04T07.

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  1. Institute of Pharmaceutical Biology, Biocenter N230, University of Frankfurt/Main, Marie-Curie-Str. 9, Frankfurt/Main, D-60439, Germany

    Dorothe Schraets, Tanja Lehmann, Theo Dingermann & Rolf Marschalek

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  1. Dorothe Schraets
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  2. Tanja Lehmann
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Correspondence to Rolf Marschalek.

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Schraets, D., Lehmann, T., Dingermann, T. et al. MLL-mediated transcriptional gene regulation investigated by gene expression profiling. Oncogene 22, 3655–3668 (2003). https://doi.org/10.1038/sj.onc.1206438

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