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A candidate gene for Drosophila genome methylation

Oncogene volume 22, pages 6301–6303 (2003)Cite this article

Abstract

Like vertebrates, the genome of Drosophila melanogaster also contains methylated cytosines. However, the enzyme(s) responsible for this methylation has been elusive. By DNA transfection and sodium bisulfite sequencing, we show here that overexpression of dDnmt2, which is the only expressed and cloned Drosophila protein consisting of motifs conserved among the DNA cytosine methyltransferases, results in genomic DNA methylation of Drosophila S2 cells. The data provide the first evidence for dDnmt2 being one candidate gene encoding the Drosophila DNA methyltransferase(s).

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Acknowledgements

This research was supported by the National Science Council and Academia Sinica, Taipei, Taiwan.

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Authors and Affiliations

  1. Institute of Molecular Biology, Academia Sinica, Nankang, Taipei, 115, ROC, Taiwan

    M Narsa Reddy, Lin-Ya Tang, Tai-Lin Lee & C-K James Shen

Authors
  1. M Narsa Reddy
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  2. Lin-Ya Tang
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  3. Tai-Lin Lee
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  4. C-K James Shen
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Correspondence to C-K James Shen.

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Narsa Reddy, M., Tang, LY., Lee, TL. et al. A candidate gene for Drosophila genome methylation. Oncogene 22, 6301–6303 (2003). https://doi.org/10.1038/sj.onc.1206650

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