Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Paper
  • Published:

Enhancement of p53 sequence-specific binding by DNA supercoiling

Oncogene volume 23, pages 2119–2127 (2004)Cite this article

Abstract

Using a new competition assay, we investigated the effect of DNA negative supercoiling on the DNA sequence-specific binding (SSDB) of human wild-type (wt) p53 protein. We found that supercoiled (sc) pBluescript DNAs with different inserted p53 target sequences were stronger competitors than a mixture of scDNA pBluescript with the given 20-mer target oligodeoxynucleotide. ScDNAs were always better competitors than their linearized or relaxed forms. Two DNAs with extruded cruciforms within the target sequence were the best competitors; removal of the cruciforms resulted in a decrease of competitor strength. In contrast to the full-length wt p53, the deletion mutant p53CΔ30 and the p53 core domain (93–312 aa) showed no enhancement of p53 SSDB to scDNA, suggesting that, in addition to the p53 core domain, the C-terminal was involved in this binding. We conclude that cruciforms and DNA bends contribute to the enhancement of p53 SSDB to scDNA and that the DNA supercoiling is an important determinant in the p53 sequence-specific binding. Supercoiling may thus play a significant role in the complex p53-regulatory network.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on SpringerLink
  • Instant access to the full article PDF.

USD 39.95

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6

Similar content being viewed by others

Abbreviations

scDNA:

supercoiled DNA

lin:

linear DNA

oligo:

oligodeoxynucleotide

oligo-t:

oligodeoxynucleotide with p53 target sequence

SK-:

plasmid pBluescript SK-

SCS:

supercoil-selective

SSDB:

sequence-specific DNA binding

fl:

full length

wt:

wild type

CON:

consensus sequence

References

  • Arrowsmith CH and Morin P . (1996). Oncogene, 12, 1379–1385.

  • Ayed A, Mulder FA, Yi GS, Lu Y, Kay LE and Arrowsmith CH . (2001). Nat. Struct. Biol., 8, 756–760.

  • Bates AD and Maxwell A . (1993). DNA Topology. Oxford University Press: New York.

    Google Scholar 

  • Brazda V, Palecek J, Pospisilova S, Vojtesek B and Palecek E . (2000). Biochem. Biophys. Res. Commun., 267, 934–939.

  • Brazdova M, Palecek J, Cherny DI, Billova S, Fojta M, Pecinka P, Vojtesek B, Jovin TM and Palecek E . (2002). Nucleic Acids Res., 30, 4966–4974.

  • Cherny DI, Striker G, Subramaniam V, Jett SD, Palecek E and Jovin TM . (1999). J. Mol. Biol., 294, 1015–1026.

  • el-Deiry WS, Kern SE, Pietenpol JA, Kinzler KW and Vogelstein B . (1992). Nat. Genet., 1, 45–49.

  • Espinosa JM and Emerson BM . (2001). Mol. Cell, 8, 57–69.

  • Fojta M, Kubicarova T, Vojtesek B and Palecek E . (1999). J. Biol. Chem., 274, 25749–25755.

  • Gohler T, Reimann M, Cherny D, Walter K, Warnecke G, Kim E and Deppert W . (2002). J. Biol. Chem., 8, 8.

  • Gottifredi V, Shieh S, Taya Y and Prives C . (2001). Proc. Natl. Acad. Sci. USA, 98, 1036–1041.

  • Hupp TR . (1999). Cell Mol. Life Sci., 55, 88–95.

  • Hupp TR, Lane DP and Ball KL . (2000). Biochem. J., 352 (Part 1), 1–17.

  • Jagelska E, Brazda V, Pospisilova S, Vojtesek B and Palecek E . (2002). J. Immunol. Methods, 267, 227.

  • Janus F, Albrechtsen N, Dornreiter I, Wiesmuller L, Grosse F and Deppert W . (1999a). Cell Mol. Life Sci., 55, 12–27.

  • Janus F, Albrechtsen N, Knippschild U, Wiesmuller L, Grosse F and Deppert W . (1999b). Mol. Cell. Biol., 19, 2155–2168.

  • Jayaraman L, Moorthy NC, Murthy KG, Manley JL, Bustin M and Prives C . (1998). Genes Dev., 12, 462–472.

  • Jayaraman L and Prives C . (1999). Cell Mol. Life Sci., 55, 76–87.

  • Jett SD, Cherny DI, Subramaniam V and Jovin TM . (2000). J. Mol. Biol., 299, 585–592.

  • Kim E, Albrechtsen N and Deppert W . (1997). Oncogene, 15, 857–869.

  • Kim E, Rohaly G, Heinrichs S, Gimnopoulos D, Meissner H and Deppert W . (1999). Oncogene, 18, 7310–7318.

  • Krasilnikov AS, Podtelezhnikov A, Vologodskii A and Mirkin SM . (1999). J. Mol. Biol., 292, 1149–1160.

  • Lee S, Cavallo L and Griffith J . (1997). J. Biol. Chem., 272, 7532–7539.

  • Levine AJ . (1997). Cell, 88, 323–331.

  • Mazur SJ, Sakaguchi K, Appella E, Wang XW, Harris CC and Bohr VA . (1999). J. Mol. Biol., 292, 241–249.

  • McKinney K and Prives C . (2002). Mol. Cell. Biol., 22, 6797–6808.

  • Murchie AIH and Lilley DMJ . (1992). Methods Enzymol., 211, 158–180.

  • Nagaich AK, Zhurkin VB, Durell SR, Jernigan RL, Appella E and Harrington RE . (1999). Proc. Natl. Acad. Sci. USA, 96, 1875–1880.

  • Nagaich AK, Zhurkin VB, Sakamoto H, Gorin AA, Clore GM, Gronenborn AM, Appella E and Harrington RE . (1997). J. Biol. Chem., 272, 14830–14841.

  • Palecek E . (1991). Crit. Rev. Biochem. Mol. Biol., 26, 151–226.

  • Palecek E, Brazdova M, Brazda V, Palecek J, Billova S, Subramaniam V and Jovin TM . (2001). Eur. J. Biochem., 268, 573–581.

  • Palecek E, Brazdova M, Cernocka H, Vlk D, Brazda V and Vojtesek B . (1999). Oncogene, 18, 3617–3625.

  • Palecek E, Vlk D, Stankova V, Brazda V, Vojtesek B, Hupp TR, Schaper A and Jovin TM . (1997). Oncogene, 15, 2201–2209.

  • Pearson CE, Zorbas H, Price GB and Zannis-Hadjopoulos M . (1996). J. Cell. Biochem., 63, 1–22.

  • Peter BJ, Ullsperger C, Hiasa H, Marians KJ and Cozzarelli NR . (1998). Cell, 94, 819–827.

  • Pospisilova S, Brazda V, Amrichova J, Kamermeierova R, Palecek E and Vojtesek B . (2000). J. Immunol. Methods, 237, 51–64.

  • Stros M and Muselikova E . (2000). J. Biol. Chem., 275, 35699–35707.

  • van Holde K and Zlatanova J . (1994). BioEssays, 16, 59–68.

  • Yagil G . (1991). Crit. Rev. Biochem. Mol. Biol., 26, 475–559.

  • Yakovleva T, Pramanik A, Kawasaki T, Tan-No K, Gileva I, Lindegren H, Langel U, Ekstrom TJ, Rigler R, Terenius L and Bakalkin G . (2001). J. Biol. Chem., 276, 15650–15658.

  • Zhou H, Zhang Y, Ou-Yang Z, Lindsay SM, Feng XZ, Balagurumoorthy P and Harrington RE . (2001). J. Mol. Biol., 306, 227–238.

Download references

Acknowledgements

This work was supported by grants of the Grant Agency of the Czech Republic 301/00/D001 to VB, and of the GAASCR Nos. B5004203 to VB, and Z5004920, A4004110 and COST OC D21.002 to EP.

Author information

Authors and Affiliations

  1. Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, 612 65, Czech Republic

    Emil Paleček, Václav Brázda, Eva Jagelská, Petr Pečinka, Lenka Karlovská & Marie Brázdová

Authors
  1. Emil Paleček
    View author publications

    Search author on:PubMed Google Scholar

  2. Václav Brázda
    View author publications

    Search author on:PubMed Google Scholar

  3. Eva Jagelská
    View author publications

    Search author on:PubMed Google Scholar

  4. Petr Pečinka
    View author publications

    Search author on:PubMed Google Scholar

  5. Lenka Karlovská
    View author publications

    Search author on:PubMed Google Scholar

  6. Marie Brázdová
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to Emil Paleček.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Paleček, E., Brázda, V., Jagelská, E. et al. Enhancement of p53 sequence-specific binding by DNA supercoiling. Oncogene 23, 2119–2127 (2004). https://doi.org/10.1038/sj.onc.1207324

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue date:

  • DOI: https://doi.org/10.1038/sj.onc.1207324

Keywords

This article is cited by

Search

Advanced search

Quick links