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Isolation and characterization of a novel gene CLUAP1 whose expression is frequently upregulated in colon cancer

Oncogene volume 23, pages 9289–9294 (2004)Cite this article

An Erratum to this article was published on 16 June 2005

Abstract

To disclose mechanisms of colorectal carcinogenesis and identify novel diagnostic markers and drug targets for treatment of these tumors, we previously analysed the expression profiles of 11 colorectal cancers using a genome-wide cDNA microarray containing 23 040 genes. Among the genes commonly transactivated in the cancers, we identified a novel human gene, which we termed CLUAP1 (clusterin-associated protein 1). It encodes a nuclear protein of 413 amino acids containing a coiled-coil domain. To investigate its function, we searched for CLUAP1-interacting proteins using yeast two-hybrid system and identified nuclear Clusterin. Expression of CLUAP1 was gradually increased in the late S to G2/M phases of cell cycle and it returned to the basal level in the G0/G1 phases. Suppression of this gene by short interfering RNAs resulted in growth retardation in the transfected cells. These data provide better understanding of colorectal carcinogenesis, and inactivation of CLUAP1 may conceivably serve in the future as a novel therapeutic intervention for treatment of colon cancer.

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Acknowledgements

We are grateful to Ms Yuka Yamane-Tanaka, Tae Makino-Nagao, Yumi Nakajima, and Yoshika Sakamoto for technical assistance, and Drs Ryuji Hamamoto, Kazutaka Obama, and Natini Jinawath for helpful discussions. This work was supported in part by Research for the Future Program Grant (00L01402) from the Japan Society for the Promotion of Science.

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Authors and Affiliations

  1. Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai Minato-ku, Tokyo, 108-8639, Japan

    Meiko Takahashi, Yusuke Nakamura & Yoichi Furukawa

  2. Department of Internal Medicine, Shin Kong Wu Ho-Su Memorial Hospital, 95 Wen Chang Road, Taipei, 11160, Taiwan

    Yu-Min Lin

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  1. Meiko Takahashi
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  2. Yu-Min Lin
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  3. Yusuke Nakamura
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  4. Yoichi Furukawa
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Correspondence to Yoichi Furukawa.

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Takahashi, M., Lin, YM., Nakamura, Y. et al. Isolation and characterization of a novel gene CLUAP1 whose expression is frequently upregulated in colon cancer. Oncogene 23, 9289–9294 (2004). https://doi.org/10.1038/sj.onc.1208100

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