Abstract
Parafibromin (PF) is a 531-amino acid protein encoded by HRPT2, a putative tumor suppressor gene recently implicated in the autosomal-dominant hyperparathyroidism-jaw tumor familial cancer syndrome and sporadic parathyroid carcinoma. To investigate effects of PF's overexpression on cell proliferation, we performed assays in four different cell lines. The transient overexpression of PF inhibited cell growth in HEK293 and NIH3T3 cells, but enhanced cell growth in the SV40 large T antigen-expressing cell lines such as 293FT and COS7 cells. In 293FT cells, PF was found to interact with SV40 large T antigen and its overexpression promoted entry into the S phase, implying that the interaction enhanced progression through the cell cycle. The tumor suppressor protein PF acts as a positive regulator of cell growth similar to an oncoprotein in the presence of SV40 large T antigen.
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Acknowledgements
We thank Dr BT Teh for anti-PF antibody. This work was supported in part by a grant-in-aid for scientific research from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
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Iwata, T., Mizusawa, N., Taketani, Y. et al. Parafibromin tumor suppressor enhances cell growth in the cells expressing SV40 large T antigen. Oncogene 26, 6176–6183 (2007). https://doi.org/10.1038/sj.onc.1210445
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DOI: https://doi.org/10.1038/sj.onc.1210445
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