Abstract
WHEN preparing the lecithin-cholesterol suspension required for the reaction of Murata (Jap. Zeit. für Derm. u. Urol., 22, No. 11; 1922; Sci. Reports Japanese Gov. Inst. Infect. Dis., vol. 2; 1923), I noticed that the most effective suspension was one which was free from visible suspended particles when freshly prepared; though the converse—that any truly colloidal suspension was suitable—was not true. The author did not note the point among his elaborate directions. He directed that the suspension should be used after standing about twenty minutes. Since at the end of this time the suspension begins to show a faint nacreous opalescence which is not removed by filtration through ordinary filter-paper, it is possible that the reaction depends in some way upon a change of state from the truly colloidal to the condition of a coarse suspension. It may be remarked that for the Wassermann antigen containing the same components an approximately colloidal state is not requisite.
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NICOL, H. Swirl Opalescence. Nature 123, 491–492 (1929). https://doi.org/10.1038/123491a0
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DOI: https://doi.org/10.1038/123491a0
