Abstract
Recent studies have shown that functional variations in clock genes, which generate circadian rhythms through interactive positive/negative feedback loops, contribute to the development of circadian rhythm sleep disorders in humans. Another potential candidate for rhythm disorder susceptibility is casein kinase I epsilon (CKIɛ), which phosphorylates clock proteins and plays a pivotal role in the circadian clock. To determine whether variations in CKIɛ induce vulnerability to human circadian rhythm sleep disorders, such as delayed sleep phase syndrome (DSPS) and non-24-h sleep–wake syndrome (N-24), we analyzed all of the coding exons of the human CKIɛ gene. One of the variants identified encoded an amino-acid substitution S408N, eliminating one of the putative autophosphorylation sites in the carboxyl-terminal extension of CKIɛ. The N408 allele was less common in both DSPS (p=0.028) and N-24 patients (p=0.035) compared to controls. When DSPS and N-24 subjects were combined, based on an a priori prediction of a common mechanism underlying both DSPS and N-24, the inverse association between the N408 allele and rhythm disorders was highly significant (p=0.0067, odds ratio=0.42, 95% confidence interval: 0.22–0.79). In vitro kinase assay revealed that CKIɛ with the S408N variation was ∼1.8-fold more active than wild-type CKIɛ. These results indicate that the N408 allele in CKIɛ plays a protective role in the development of DSPS and N-24 through alteration of the enzyme activity.
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Acknowledgements
We wish to express appreciation for the cooperation of all the participants. We thank Dr Mariko Nagao, Dr Keiko Kim, and Dr Yoshihisa Kudo for recruiting patients. Technical contributions by Mr Eiichi Yamada, Ms Kyoko Ohnishi, and Mr Masakazu Kinoshita are gratefully acknowledged. This work was supported financially by the Ministry of Health and Welfare, and the Ministry of Education, Science, Sports, and Culture (10557089, 11233206, 12470198), and Saitama Medical School.
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Takano, A., Uchiyama, M., Kajimura, N. et al. A Missense Variation in Human Casein Kinase I Epsilon Gene that Induces Functional Alteration and Shows an Inverse Association with Circadian Rhythm Sleep Disorders. Neuropsychopharmacol 29, 1901–1909 (2004). https://doi.org/10.1038/sj.npp.1300503
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DOI: https://doi.org/10.1038/sj.npp.1300503
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