Abstract
OSMIUM tetroxide at 18s. a gram is the most expensive substance used in biological research. It is principally used in the Kolatchew and Weigl methods for the Golgi apparatus, and in the F. W. A. and Champy methods for mitochondria. The nearest cheap substitutes for Weigl and Kolatchew methods are the formalin-silver methods of Cajal and Da Fano. The trouble with these latter methods is that there is usually distortion and often incrustation of the Golgi bodies, banana-shaped elements, for example, often becoming beads, or spheres, and different from what can be seen intra vitam. The general fixation is often very poor.
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References
Z. wiss. Mikr., (1930).
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GATENBY, J. A Satisfactory Substitute for the Osmium Tetroxide Golgi Apparatus Methods. Nature 137, 619 (1936). https://doi.org/10.1038/137619a0
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DOI: https://doi.org/10.1038/137619a0
