Developmental Forms of the Virus of Lympho-granuloma Inguinale (Climatic Bubo)

Abstract

IT is now generally agreed that in lesions produced by the virus of lymphogranuloma inguinale (climatic bubo) there can be seen small elementary granules which by staining and by differential nitration appear to be 100–175 mµ in size. These granules almost certainly represent the etiological agent of the disease. In smears from infected mouse brains these granules may occur (1) diffusely scattered outside the cell, in the form of colony-like masses ; (2) within the cell cytoplasm, when they may be surrounded by a definite membrane ; or (3) closely adherent to ruptured cells. The granules are stained blue by Castaneda's stain, blue with Victoria B, and reddish-violet by Giemsa's method.