Determination of rH in Endocrine Extracts of a Protein Nature

Abstract

WE have begun to study systematically extracts of endocrine glands containing hormones of a protein nature, especially extracts of the anterior pituitary lobe (ALH) of cattle. For technical reasons very dilute extracts were used: 1 gm. of the dry powder, previously kept in vacuo, was shaken for 4 hours with 20 ml. of a 0.0001 N NH₄Cl solution and then filtered. The pH. of the filtrate was kept at about 6.2.