Abstract
IN treating plasma from time-expired blood according to the method described by McFarlane1 for the removal of lipoid from human serum, it was found that in addition to extracting lipoid from combination with plasma protein, the flbrinogen was also removed. The quantitative removal of fibrinogen was effected by adjusting the plasma with citric acid to pH 5.4 prior to freezing to â 30° C. with excess ether. Solutions of extracted plasma protein obtained in this manner, after the removal of the dissolved ether and readjustment to p.H 7.0, have provided a satisfactory transfusion fluid, remaining crystal clear for more than two years2.
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References
McFarlane, A. S., Nature, 140, 139 (1942).
Kekwick, R. A., McFarlane, A. S., and Mackay, M. E., unpublished.
Cohn, E. J., et al., J. Clin. Invest, 23, No. 4 (1944).
Brit. Patent Application 31199/45.
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KEKWICK, R., MACKAY, M. & RECORD, B. Fractionation of Human Plasma with Ether. Nature 157, 629 (1946). https://doi.org/10.1038/157629a0
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DOI: https://doi.org/10.1038/157629a0
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