Abstract
IN our earliest studies on the tryptophanase complex1 active non-viable suspensions of E. coli were used. These had been sterilized by shaking with chloroform, 1 volume to 2 volumes of suspension. The mixture was shaken vigorously to 200 r.p.m. for 4 hours.
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References
Biochem. J., 29, 1918 (1935).
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DAWES, E., DAWSON, J. & HAPPOLD, F. Preparation of Cell-free Tryptophanase. Nature 159, 99 (1947). https://doi.org/10.1038/159099a0
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DOI: https://doi.org/10.1038/159099a0
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