Abstract
In a study of creatinine metabolism in the rat, to be published elsewhere, creatinine has been identified qualitatively by means of the technique of partition chromatography on paper strips developed by Consden, Gordon and Martin1. The apparatus described by Dent2 has been used, with the further refinement of accurate temperature control obtained by immersing the outer glass container in a water thermostat at 15 ± 0·05°C. Solutions containing c. 20–30 γ of creatinine were pipetted on to the paper strips, and after treatment with an appropriate solvent, for example, collidine for 24 hours, the creatinine bands were located by spraying with alkaline sodium picrate.
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References
Consden, R., Gordon, A. H., and Martin, A. J. P., Biochem. J., 38, 224 (1944).
Dent, C. E., Lancet, ii, 637 (1946).
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MAW, G. Identification of Creatine by Partition Chromatography. Nature 160, 261 (1947). https://doi.org/10.1038/160261a0
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DOI: https://doi.org/10.1038/160261a0
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