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Use of Certain Growth-Inhibitors as Tools in Metabolic Investigations

Abstract

CULTURES of Saccharomyces carlsbergensis grown in the presence of sub-optimal amounts of vitamin B6, biotin, or pantothenic acid, and containing in each case less than one tenth of the normal quantity per mgm. of the growth-factor, possessed reduced rates of fermentation, which could be increased within a few minutes at most by addition of the latter. A rapid and marked stimulation was produced also by the addition of sodium azide in suitable concentrations ; typical results are shown in the accompanying figure.

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References

  1. Lipmann, F., "Advances in Enzymology", 1, 121 (1941).

  2. Brockmann, M. C., and Stier, T. J. B., J. Cell. and Comp. Physiol., 29, 159 (1947).

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PENNINGTON, R. Use of Certain Growth-Inhibitors as Tools in Metabolic Investigations. Nature 162, 661 (1948). https://doi.org/10.1038/162661a0

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