A Simple Apparatus for Adding Seitz-Filtered Components Aseptically to Embryo Culture Media

Abstract

IN attempting to culture excised immature plant embryos, it is frequently desirable to avoid autoclaving the nutrilite components (for example, malt extract). These are usually Seitz-filtered separately and transferred by pipette to the individual culture vials containing the previously autoclaved sugar and inorganic components of the medium. To workers unskilled in bacteriological methods, this represents a tedious and time-consuming operation.