Abstract
FERTILIZED mouse ova have been cultivated in vitro, and their development filmed, by Friedrich-Freksa and Kuhl1, who used as medium a clot of guinea pig plasma and mouse embryo extract containing segments of Fallopian tube2. Like Chang3, I have been working on ovum culture with a view to transplantation of ova. Chang has used rabbits, with serum as a culture medium ; I have chosen to use mice, as more readily available, and because (like most domestic animals) they have naked eggs. Seeking a medium readily prepared in large quantities, I have tried saline hen-egg extracts.
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References
Kuhl, W., Abh. senckenberg. naturf. Ges., 456, 1 (1941).
Kuhl, W. (personal communication, 1947).
Chang, M. C., Nature, 159, 602 (1947).
Chang, M. C., Nature, 161, 978 (1948).
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HAMMOND, J. Recovery and Culture of Tubal Mouse Ova. Nature 163, 28–29 (1949). https://doi.org/10.1038/163028b0
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DOI: https://doi.org/10.1038/163028b0
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