Abstract
In a previous paper1 from these laboratories, it was shown that the mechanism for the Dische2 diphenylamine reaction for deoxyribonucleic acid depends upon the conversion by acid treatment of the depxyribose component into w-hydroxy lævulinic aldehyde, which then coupled with diphenylamine to give a blue dye with a characteristic absorption band. Extension of this work with Dr. Ethel Teece has led to the isolation in 10 per cent yield of an amorphous blue complex possessing the characteristic band.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Deriaz R. E., Stacey, M., Teece, E. G., and Wiggins, L. F., Nature, 157, 740 (1946).
Dische, Z., Microchemie, 8, 4 (1930).
Feulgen, R., and Rosenbeck, H., Z. physiol. Chem., 135, 203 (1924).
Danielli, J. F., Symposia Soc. Exp. Biol., 1, 101 (1947).
Stedman, E., and Stedman, E., Biochem. J., 43, xxiii (1948).
Tobie, W. C., Indust. Eng. Chem., 14 (5), 405 (1942).
Wieland H., and Scheuing, G., Ber., 54, 2527 (1921); see also Rumpf, P., Ann. Chim., 3, 327 (1935).
Widström, G., Biochem. Z., 199, 298 (1928).
Gulland, J. M., and Jordan, D. O., J. Chem. Soc., 1129 (1947).
Stedman, E., and Stedman, E., Symp. Soc. Exp. Biol., 1, 232 (1947), and previous publications.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
OVEREND, W., STACEY, M. Mechanism of the Feufgen Nucleal Reaction. Nature 163, 538–540 (1949). https://doi.org/10.1038/163538b0
Issue date:
DOI: https://doi.org/10.1038/163538b0
This article is cited by
-
Untersuchungen über altersabhängige Veränderungen der Säurelabilität des Nucleoproteids
Virchows Archiv B Cell Pathology (1969)
-
Polyploidy and radiosensitivity in wheat and barley
Genetica (1962)
-
Trichloracetic Acid and Feulgen Staining
Nature (1951)
-
Application of the Dische Diphenylamine Reaction to Pyrimidine Deoxynucleosides
Nature (1951)