Abstract
IN a recent report by Gilbert and Stacey1 on the constitution of a bacterial levan, these authors state that a convenient chromatographic technique for the quantitative analysis of tetra-, tri- and di-methyl fructoses had so far eluded them. We have devised a partition chromatogram giving quantitative results in the analysis of mixtures of 1 : 3 : 4 : 6-tetramethyl, 1 : 3 : 4-trimethyl and 3 : 4-dimethyl fructoses which is applicable to the study of methylated fructosans based on these sugars. (3 : 4 : 6-Trimethyl fructose has also been examined ; but certain difficulties, not yet overcome, arise in analytical separations involving this sugar.)
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References
Gilbert, V. E., and Stacey, M., J. Chem. Soc., 1560 (1948).
Bell, D. J., J. Chem Soc., 473 (1944).
Brown, F., Hirst, E. L., Hough, L., Jones, J. K. N., and Wadman, H., Nature, 161, 720 (1948).
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BELL, D., PALMER, A. Quantitative Chromatographic Analysis of Tetra-, Tri- and Di-methyl Fructoses. Nature 163, 846 (1949). https://doi.org/10.1038/163846a0
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DOI: https://doi.org/10.1038/163846a0
