Abstract
THE simplest method of preparing bacteria for observation in the electron microscope is to deposit them from suspension on to a collodion membrane. However, when developmental changes are to be followed, it is preferable to devise a means of mounting them in a state as near as possible to their normal mode of growth. We have previously described one such method1 in which a collodion solution is poured over the growth medium and allowed to dry. When floated off in water, the resulting film carries with it the majority of bacteria. A similar method has been described by Hillier and Baker2.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to the full article PDF.
USD 39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Brieger, E. M., Crowe, G. R., and Cosslett, V. E., Nature, 160, 864 (1947).
Hillier, J., and Baker, R. F., J. Bact., 52, 411 (1946).
Brieger, E. M., and Fell, H., J. Hyg. (Camb.), 44, 158 (1945).
Unpublished.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
BRIEGER, E., COSSLETT, V. Preparation of Bacteria for Electron Microscopy. Nature 164, 352–353 (1949). https://doi.org/10.1038/164352a0
Issue date:
DOI: https://doi.org/10.1038/164352a0
