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Separation of Substances Related to Ascorbic Acid

Nature volume 164, pages 479–480 (1949)Cite this article

Abstract

THE method of paper partition chromatography has in recent years been widely used for the separation, detection and estimation of closely related compounds. We thought it of interest to determine to what extent it could be used to differentiate l-ascorbic acid from some of its isomers and from other nosely related dienols. Simple titration methods using 2 : 6 dichlorophenolindophenol give a reliable estimate of the vitamin content of most of the common foodstuffs, as shown by the close correspondence between chemical and biological results1-3. A method proposed by Roe and Kuether4, which is based on the formation of a coloured condensation product of 2: 3 diketogulonic acid with 2: 4 dinitro-phenylhydrazone, also gives results which are generally in agreement with those of the indophenol method.

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Authors and Affiliations

  1. Low Temperature Station for Research in Biochemistry and Biophysics, Cambridge

    L. W. MAPSON &  S. M. PARTRIDGE

Authors
  1. L. W. MAPSON
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  2. S. M. PARTRIDGE
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MAPSON, L., PARTRIDGE, S. Separation of Substances Related to Ascorbic Acid. Nature 164, 479–480 (1949). https://doi.org/10.1038/164479a0

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