Electrophoresis of Proteins in Agar Jelly

Abstract

THE ease with which ionophoretic separations of amino-acids and lower peptides can be carried out in a rectangular slab of silica jelly¹ suggested that a similar technique might be useful for separating the proteins. The first problem was to find a convenient jelly which would allow the free migration of large molecules. The unsuitability of silica jelly for this purpose was shown when an attempt was made to cause hæmoglobin to migrate into its surface. Unfortunately, the protein became fixed less than 1 mm. from the surface of entry. A 1 per cent agar jelly, on the other hand, was found to allow the protein to migrate.