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Use of Pyridine in the De-ionization of Solutions for Paper Chromatography

Abstract

Partridge1 and Westall2 have stressed the need for the complete removal of electrolytes in preparing solutions for paper chromatography when the subsequent development of the chromatogram involves the use of ammoniacal silver nitrate. The use of ion-exchange columns—'Zeo-Karb 215' and 'Deacidite E'—for this purpose in the routine analysis of dilute solutions of sugars in Ringer phosphate incubates proved slow and uncertain in our hands. The following procedure, however, based on the solubility of sugars and the insolubility of inorganic salts in pyridine, has given a consistent elimination of salt effects in our chromatographic studies, and is well suited to routine investigations.

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References

  1. Partridge, S. M., Biochem. J., 42, 238 (1948).

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  2. Westall, R. G., Biochem. J., 42, 249 (1948).

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  3. Jermyn, M. A., and Isherwood, F. A., Biochem. J., 44, 402 (1949).

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MALPRESS, F., MORRISON, A. Use of Pyridine in the De-ionization of Solutions for Paper Chromatography. Nature 164, 963 (1949). https://doi.org/10.1038/164963a0

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