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Aeration of Culture Media

Abstract

IN biological work, oxygen has often to be supplied to an organism by bubbling air, or some other oxygen-containing gas, through the culture medium. The rate at which oxygen is available for consumption by the organism is frequently limited by the rate at which oxygen dissolves from the gas phase. Although this has been realized1, there has been little attention given to this point in the literature. An easy method of assessing the effect of aeration has been developed, using a polarograph to measure the concentration of dissolved oxygen2.

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References

  1. Olson and Johnson, J. Bact., 57, 235 (1949).

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  2. Petering and Daniels, J. Amer. Chem. Soc., 60, 2796 (1938).

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  3. Sherwood, T. K., “Absorption and Extraction”, 64 (McGraw-Hill, 1937).

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WISE, W. Aeration of Culture Media. Nature 165, 249 (1950). https://doi.org/10.1038/165249a0

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