Quantitative Determination of Dehydroisoandrosterone in Pure Solutions and in Urine Extracts

Abstract

2 ml. of pure concentrated sulphuric acid are added drop by drop to 0.1 ml. of a solution of dehydroisoandrosterone in ethyl alcohol or of an extract containing 5–50 γ of that substance in a test tube. The mixture is heated to 60° C. for 25 min. and then cooled in ice water. The chilling is discontinued, and 5 ml. ethyl ether (water- and peroxide-free) are added slowly while the mixture is thoroughly stirred with a glass rod.