Abstract
PRE-TREATMENT with 8-oxyquinoline (0.002 mol. per litre for four hours) of root meristems followed by squashing in acetic orceine gives chromosome pictures of exceptional clearness. The chromosomes are contracted as after cold or c-mitotic treatment. At the same time the metaphase chromosomes remain in their original position within the equatorial plate, although the spindle does not interfere with their spreading at the squashing. Satellites and secondary constrictions are conspicuous, and help the analysis of idiograms. Prophase chromosomes often show heterochromatic differentiations. The knobs of rye chromosomes are made quite conspicuous in mitotic prophase.
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References
Botaniska Notiser, 176 (1947).
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TJIO, J., LEVAN, A. Quadruple Structure of the Centromere. Nature 165, 368 (1950). https://doi.org/10.1038/165368a0
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DOI: https://doi.org/10.1038/165368a0
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