Abstract
THE deep magenta colour given by ergothioneine on treatment with buffered diazotized sulphanilic acid followed by 10 N sodium hydroxide has been used for the determination of ergothioneine in blood1. The specificity of this reaction comes into question and has been considered by Hunter2.
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References
Hunter, G., Biochem. J., 22, 4 (1928); Canad. J. Res., 27, E, 230 (1949).
Hunter, G., J. Chem. Soc., 2343 (1930).
Lawson, A., Morley, H. V., and Woolf, L. I., Biochem. J., 47, 513 (1950).
Cf. Fargher, R. G., and Pyman, F. L., J. Chem. Soc., 217 (1919).
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LAWSON, A., MORLEY, H. & WOOLF, L. Specificity and Mechanism of the Hunter Reaction for Ergothioneine. Nature 167, 82–83 (1951). https://doi.org/10.1038/167082a0
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DOI: https://doi.org/10.1038/167082a0
