Abstract
IN recent studies we were concerned with the detection of traces of nor-adrenaline in preparations of adrenaline derived from natural sources. Colorimetric methods1,2 proved to be of limited value with low concentrations of nor-adrenaline; but excellent results were obtained with paper chromatography, using modifications of the methods described by James and others3,4. The adrenaline samples are dissolved in methanol containing a minimum quantity of hydrochloric acid, and then transferred to paper strips for descending chromatography, or to circular disks of filter paper for chromatography by Rutter's technique5. Water-saturated phenol is used as the developing solvent in an atmosphere of hydrochloric acid4. The paper chromatograms are then dried and sprayed with an appropriate colour reagent.
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References
Von Euler, U. S., and Hamburg, U., Acta Physiol. Scand., 19, 74, (1949).
Auerbach, M. E., and Angell, E., Science, 109, 537 (1949).
James, W. O., Nature, 161, 851 (1948).
Goldenberg, M., Faber, M., Alston, E. J., and Chargaff, E. C., Science, 109, 534 (1949).
Rutter, L., Analyst, 75, 37 (1950).
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GLAZKO, A., DILL, W. A Colour Reaction distinguishing between Adrenaline and nor-Adrenaline on Paper Chromatograms. Nature 168, 32 (1951). https://doi.org/10.1038/168032b0
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DOI: https://doi.org/10.1038/168032b0
