Abstract
FOR the demonstration of the Golgi material in animal cells by the formalin silver nitrate method, Cajal's uranium nitrate1, Da Fano's cobalt nitrate2,3 and Aoyama's cadmium chloride4 techniques are employed. As a result of careful experiments, I have found that the following new fixative gives very good results: cupric chloride (1 per cent in distilled water), 85 c.c.; neutral formol, 15 c.c.
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References
Cajal, R. Y., Trab. Lab. Invest. Biol. Madrid, 12, 127 (1914).
Da Fano, C., J. Roy. Mik. Soc., 40, 157 (1920).
Da Fano, C., J. Physiol., 54, 113 (1920).
Aoyama, F., Z. Wiss. Mikr., 46, 489 (1929).
Gatenby, J. B., and Beams, H. W., “The Microtomists' Vademecum” (Bolles-Lee) (11th edit., 1950), Chapter 32, see p. 413.
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SRIVASTAVA, D. A New Method for the Demonstration of the Golgi Material. Nature 169, 549–550 (1952). https://doi.org/10.1038/169549b0
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DOI: https://doi.org/10.1038/169549b0
