CD34⁺/CD41a⁺ cells best predict platelet recovery after autologous peripheral blood stem cell transplantation

Abstract

Reliable markers for megakaryocytic reconstitution after peripheral blood stem cell transplantation (PBSCT) have not been established. To determine a convenient and reliable predictor, we measured the number of megakaryocyte progenitor cells in PBSC grafts by clonogenic and flow cytometric assays. Seventeen patients with hematological and solid malignancies were included in this study. For the clonogenic assay, we used thrombopoietin (TPO) as a growth factor to evaluate the maximum number of megakaryocyte progenitor cells. Using a flow cytometric assay, we examined the expression of platelet glycoproteins on CD34⁺ cells to count the number of megakaryocyte progenitor cells. We used buffer containing EDTA to prevent platelet adhesion to CD34⁺ cells and selected CD34⁺ cells by immunomagnetic beads. The best correlation was observed between the number of CD34⁺/CD41a⁺ cells and the time to platelet recovery (P = 0.0205), rather than the total number of CD34⁺ cells. In addition, a close correlation was observed between the number of CD34⁺/CD41a⁺ cells and colony-forming unit megakaryocyte (CFU-MK) (P = 0.0018). These observations suggest that the number of CD34⁺/CD41a⁺ cells is the best predictor for platelet reconstitution after PBSCT.