Abstract
IT has recently been demonstrated that reasonably homogeneous preparations of cell nuclei can be isolated in good yield from rat or mouse liver1. By means of centrifugal fractionation of liver homogenates prepared in an aqueous medium (0.25 M sucrose – 0.0018 M calcium chloride), it has been possible to obtain 70–90 per cent of the nuclei with a minimum of cytological alteration in preparations containing by direct count less than 1 per cent of the intact cells of the tissue and less than 0.5 per cent of the free mitochondria of the homogenates.
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References
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Hogeboom, G. H., and Schneider, W. C. (unpublished experiments).
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HOGEBOOM, G., SCHNEIDER, W. Synthesis of Diphosphopyridine Nucleotide by Cell Nuclei isolated in Aqueous Media. Nature 170, 374 (1952). https://doi.org/10.1038/170374a0
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DOI: https://doi.org/10.1038/170374a0
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