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Peripheral Blood Stem Cells

Mathematical model of peripheral blood stem cell harvest kinetics

Bone Marrow Transplantation volume 32, pages 749–757 (2003)Cite this article

Summary:

A mathematical model of peripheral blood stem cell harvests was developed, taking two new parameters R (number of recruited cells/minute) and Ef (efficiency of collection) into consideration in addition to concentrations and collected amounts of cells. This model was tested on 241 harvest procedures in cancer patients (chemotherapy+G-CSF stimulation), donors of allogeneic PBSC, and platelet donors, using different collection procedures, with a Cobe Spectra Cell separator. The relationships between preapheresis concentrations, R, Ef and harvested amounts of cells were complex, and different for different harvest procedures and populations of donors. However, invariably, recruitment played an important role and contributed significantly to the final harvest in all types of cells studied. For example, for the patient group, mean recruitment was 1.3 × 106 CD34+ cells/min and the amount of recruited cells corresponded to 65% of all collected cells. Recruitment was significantly influenced by pretreatment with chemo-therapy and/or radiotherapy. The mean recruitment values for the subgroups with limited, moderate, and extensive pretreatment were 1.65 × 106, 0.87 × 106, and 0.32 × 106 CD34+ cells released per minute, respectively. The finding of a quick and massive recruitment phenomenon may stimulate further research into hematopoiesis in order to maximize harvested cells.

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Acknowledgements

We thank all the physicians and nurses from the Department of Internal Medicine – Hematooncology, and Blood Transfusion Service, University Hospital Brno, for taking care of patients and donors, and to the staff of the Laboratory of Flow Cytometry and the Tissue Bank, University Hospital Brno, for analyses of CD34+ cells and CFU-GM, respectively. This work was supported in part by Research Grant No. MZ 00065269705 of the Ministry of Health of the Czech Republic.

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Authors and Affiliations

  1. Department of Internal Medicine – Hematooncology, University Hospital Brno, Brno, Czech Republic

    J Mayer & Z Kořístek

  2. Department of Mathematical Analysis, Faculty of Science, Masaryk University, Brno, Czech Republic

    Z Pospíšil

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  1. J Mayer
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  2. Z Pospíšil
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  3. Z Kořístek
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Correspondence to J Mayer.

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Mayer, J., Pospíšil, Z. & Kořístek, Z. Mathematical model of peripheral blood stem cell harvest kinetics. Bone Marrow Transplant 32, 749–757 (2003). https://doi.org/10.1038/sj.bmt.1704226

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