Abstract
IT has often been stated that the colour-producing reaction between creatinine and alkaline picrate does not obey Beer's law1–3. This implies that a calibration graph relating creatinine concentration to light extinction is not a straight line. Curves have been obtained with photoelectric absorptiometers using filters which pass an appreciable band of wavelengths, for example, 495–550 mµ4.
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References
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HERVEY, G. Determination of Creatinine by the Jaffé Reaction. Nature 171, 1125 (1953). https://doi.org/10.1038/1711125a0
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DOI: https://doi.org/10.1038/1711125a0
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