Abstract
A BACITRACIN polypeptide purified by charcoal chromatography was shown by ultracentrifugation to have a molecular weight of 1,400–1,5001. Recently, by a modified procedure using milder conditions, a peptide of higher antibiotic potency (about 60 units/mgm.) was isolated from a cruder starting material than was previously used. Except for higher activity, this peptide has essentially the same properties as that previously studied. This preparation was compared with a specimen of bacitracin A 2 kindly supplied by Dr. L. C. Craig, and appears to be identical with it. Ultracentrifugation of bacitracin A indicated a somewhat lower molecular weight (1,390), but the difference is probably not significant. The most potent fraction obtained from charcoal will therefore be referred to as bacitracin A.
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References
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PORATH, J. Structure of Bacitracin A . Nature 172, 871 (1953). https://doi.org/10.1038/172871a0
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DOI: https://doi.org/10.1038/172871a0
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