Abstract
FLUORIMETRY is now recognized as a very sensitive method for the quantitative determination of natural œstrogens. Unfortunately, when applying this method to urinary extracts, it was found that fluorimetric values are influenced by a large amount of non-œstrogenic fluorescent materials. We are now attacking the problem of removing this interference. This can be partly accomplished by further purifications of the extracts, either by counter-current distribution1 or by chromatography2,3.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to the full article PDF.
USD 39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Engel, L. L., Slaunwhite, W. R., Carter, P., and Nathanson, I. T., J. Biol. Chem. 185, 255 (1950).
Swyer, G. I. M., and Braunsberg, H., Proc. Soc. Endocrin., 7, 60 (1951).
Heusghem, C., Annales d'Endocrin., 13, No. 3, 479 (1952).
Kober, S., Biochem. Z., 239, 209 (1931).
Marrian, G. F., Biochem. J., 24, 435 (1930).
Veldhuis, A. H., J. Biol. Chem., 202, 107 (1953).
Bates, R. W., and Cohen, H., Endocrin., 47, 166 and 182 (1950).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
HEUSGHEM, C. Elimination of the Fluorescence of Œstrogens in Urinary Extracts by Hydrogen Peroxide. Nature 173, 1043–1044 (1954). https://doi.org/10.1038/1731043a0
Issue date:
DOI: https://doi.org/10.1038/1731043a0
