Abstract
USING a method described elsewhere1, we have been able to cut serial sections of about 250 A. thickness, and, by varying the speed of cutting successive sections, serial sections of thickness alternately about 350 A. and 150 A. There were always thin and thick areas within individual sections. On the basis of the general rule suggested by Cosslett2, that the optimum resolution obtainable from a section is of the order of one-tenth of the thickness, the thinner parts of the 150 A. sections should afford resolutions of 10 A. We have succeeded in obtaining such resolutions, as illustrated in Fig. 1.
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References
Eaves, G., Ph.D. thesis, University of Leeds (1953). Eaves, G., and Flewett, T. H., Exp. Cell Res. (in the press).
Cosslett, V. E., “Practical Electron Microscopy” (Butterworth and Co., London, 1951).
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FLEWETT, T., EAVES, G. High Resolution Electron Micrographs of Thin Tissue Sections. Nature 173, 309 (1954). https://doi.org/10.1038/173309a0
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DOI: https://doi.org/10.1038/173309a0
