Abstract
THE amounts of materials adsorbed on paper chromatograms can often be estimated directly from measurements of the optical densities of the spots produced by colour-forming reagents. In some cases, it is possible to avoid the colour-forming reaction by measuring the ultra-violet or visible absorptions of the materials themselves. Bellamy1 has shown that it is possible to examine directly the infra-red spectra of materials adsorbed on paper chromatograms, and it has now been found possible to make quantitative measurements on paper chromatograms by scanning the paper with infra-red radiation of suitable wave-lengths. This method may prove to be particularly useful for substances which do not absorb in the ultra-violet or visible regions and which do not undergo quantitative colour-forming reactions.
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References
Bellamy, L. J., J. App. Chem., 3, 421 (1953).
Jones, R. N., J. Amer. Chem. Soc., 74, 2681 (1952).
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GOULDEN, J. Quantitative Evaluation of Paper Chromatograms by Infra-red Absorption. Nature 173, 646 (1954). https://doi.org/10.1038/173646a0
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DOI: https://doi.org/10.1038/173646a0
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