Simultaneous Evaluation of Circulating Eosinophils, Polymorphonuclears and Lymphocytes by Phase-contrast Microscopy

Abstract

WHILE counting blood eosinophils, it may be very useful to determine the corresponding numbers of the other white cells in the same preparation. This can be effected in a simple and reliable way, by using phase-contrast microscopy and a modified Zollikofer's solution¹, composed of 1 ml. yellow eosin (2 per cent aqueous solution, kept in a refrigerator), 3 ml. formalin (40 per cent, neutralized with calcium carbonate) and distilled water to make up 100 ml.