Abstract
THE action of ribonuclease on living cells from various materials has recently been studied1,2. Chick fibroblasts and myoblasts in vitro are readily affected; growth of the cultures and the number of mitoses are considerably reduced2; cytoplasmic and nucleolar basophilia is diminished or suppressed and a typical modification of the chromatin, seen both with phase-contrast optics in living cells and on fixed preparations, is observed. Many nuclei (up to 95 per cent when the action of ribonuclease has been particularly intense) contain granular, or granular and filamentous, material or even are prophasic in appearance3. Even when many nuclei are showing this prophasic aspect, few—if any—other mitotic stages are encountered.
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References
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FIRKET, H., CHÈVREMONT-COMHAIRE, S. & CHÈVREMONT, M. Action of Ribonuclease on Living Cells in vitro and Synthesis of Deoxyribonucleic Acid. Nature 176, 1075–1076 (1955). https://doi.org/10.1038/1761075a0
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DOI: https://doi.org/10.1038/1761075a0
